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Chromatography Practice Exam

Chromatography Practice Exam


About Chromatography Exam

The Chromatography Practice Exam is designed to test your understanding of the principles and applications of chromatography in chemical analysis. It checks your knowledge of chromatographic techniques, system components, sample preparation, and result interpretation. This exam is useful for chemistry students, lab technicians, researchers, and professionals working in pharmaceuticals, food testing, environmental labs, and chemical industries.


Who should take the Exam?

This exam is ideal for:

  • Chemistry and biochemistry students
  • Laboratory technicians and analysts
  • Professionals in pharmaceutical, food, and environmental labs
  • Quality control and quality assurance staff
  • Researchers using chromatography for chemical separation
  • Candidates preparing for laboratory or technical certifications


Skills Required

  • Basic knowledge of chemistry and lab practices
  • Understanding of chemical mixtures and separation techniques
  • Familiarity with instruments like HPLC, GC, or TLC
  • Ability to prepare and handle samples
  • Awareness of safety and quality control in labs


Knowledge Gained

  • How chromatography is used to separate and analyze compounds
  • Understanding retention time, resolution, and peak analysis
  • Using different types of chromatography such as HPLC, GC, and TLC
  • Sample preparation and mobile/stationary phase selection
  • Identifying and solving common chromatography issues
  • Learning practical applications in real-world lab settings


Course Outline

The Chromatography Exam covers the following topics -

Domain 1 – Introduction to Chromatography
  • What chromatography is and why it is used
  • Types of chromatography and their differences


Domain 2 – Chromatographic Techniques

  • High-Performance Liquid Chromatography (HPLC)
  • Gas Chromatography (GC)
  • Thin Layer Chromatography (TLC)


Domain 3 – Instrumentation and Components

  • Mobile and stationary phases
  • Detectors, columns, and sample injectors


Domain 4 – Sample Preparation and Handling

  • Filtration, dilution, and storage
  • Avoiding contamination and ensuring accuracy


Domain 5 – Data Interpretation and Troubleshooting

  • Reading chromatograms and identifying peaks
  • Solving issues with baseline, tailing, or peak overlap


Domain 6 – Practical Applications and Lab Safety

  • Use of chromatography in different industries
  • Lab safety, documentation, and quality assurance

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